A Computational Model of Light-Sheet Fluorescence Microscopy using Physically-based Rendering

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dc.contributor.authorAbdellah, Marwanen_US
dc.contributor.authorBilgili, Ahmeten_US
dc.contributor.authorEilemann, Stefanen_US
dc.contributor.authorMarkram, Henryen_US
dc.contributor.authorSchürmann, Felixen_US
dc.contributor.editorB. Solenthaler and E. Puppoen_US
dc.date.accessioned2015-04-15T18:40:50Z
dc.date.available2015-04-15T18:40:50Z
dc.date.issued2015en_US
dc.description.abstractWe present a physically-based computational model of the light sheet fluorescence microscope (LSFM). Based on Monte Carlo ray tracing and geometric optics, our method simulates the operational aspects and image formation process of the LSFM. An extension for previous fluorescence models is developed to account for the intrinsic characteristics of fluorescent dyes in order to accurately simulate light interaction with fluorescent-tagged biological specimen. This extension was quantitatively validated against the fluorescence brightness equation and experimental spectra of different dyes. We demonstrate first results of our rendering pipeline to a simplified brain tissue model reconstructed from the somatosensory cortex of a young rat.en_US
dc.description.sectionheadersPostersen_US
dc.description.seriesinformationEG 2015 - Postersen_US
dc.identifier.doi10.2312/egp.20151038en_US
dc.identifier.pages15-16en_US
dc.identifier.urihttp://dx.doi.org/10.2312/egp.20151038en_US
dc.publisherThe Eurographics Associationen_US
dc.subjectI.3.3 [Computer Graphics]en_US
dc.subjectPicture/Image Generationen_US
dc.subjectRenderingen_US
dc.titleA Computational Model of Light-Sheet Fluorescence Microscopy using Physically-based Renderingen_US
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